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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ Filamin Antibody Monoclonal IF,IHC,IP,WB LS‑B6313

Catalog Number Size Price
LS-B6313-50 50 µl $485 
Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHCPlus™ Filamin Antibody Monoclonal IF,IHC,IP,WB LS‑B6313

Note: This antibody replaces LS-C23664
Antibody:
Mouse Monoclonal to Human Filamin
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B6313-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Filamin
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
Filamin antibody LS-B6313 is an unconjugated mouse monoclonal antibody to Filamin from human and bovine. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Filamin
Host
Mouse
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:250 - 1:1000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human platelet protein.
Specificity
Human filamin (actin-binding protein). Recognizes the 90kD fragment of filamin produced by calpain cleavage. Species cross-reactivity: Human and bovine. Does not react with Mouse or rat.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Western Blot: 1:250-1:1000. Immunoprecipitation: Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for. every 300-500muL of cell lysate (200-500 ug/mL total protein is suggested. Incubation is 1hour RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are. recommended for full length filamin or the 80kD fragment visualization. Immunofluorescence:1:50-1:200. ABC. Immunohistochemistry: foramlin-fixed paraffin-embedded sections. Requires HIER (citrate buffer). Positive control: Human, prostate or bovine skin.
Presentation
No preservatives added
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC Validated
Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

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Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human, Mouse, Rat, Guinea pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Reactivity: Mouse
Range: 1.56-100 pg/ml
Reactivity: Human
Range: 15.63-1000 pg/ml

Requested From: United States
Date Requested: 8/19/2019
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