(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:50)
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:250 - 1:1000)
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Human platelet protein.
Human filamin (actin-binding protein). Recognizes the 90kD fragment of filamin produced by calpain cleavage. Species cross-reactivity: Human and bovine. Does not react with Mouse or rat.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Western Blot: 1:250-1:1000. Immunoprecipitation: Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for. every 300-500muL of cell lysate (200-500 ug/mL total protein is suggested. Incubation is 1hour RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are. recommended for full length filamin or the 80kD fragment visualization. Immunofluorescence:1:50-1:200. ABC. Immunohistochemistry: foramlin-fixed paraffin-embedded sections. Requires HIER (citrate buffer). Positive control: Human, prostate or bovine skin.
No preservatives added
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.