Synthetic peptide located between aa108-157 of human DENND1B (Q6P3S1, NP_659414). Percent identity by BLAST analysis: Human, Chimpanzee, Bovine (100%); Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Horse, Pig, Guinea pig (92%); Dog, Rabbit (85%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
This antibody carries the LSBio 100% Guarantee.