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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ CRD‑BP / ZBP1 / IGF2BP1 Antibody aa19‑32 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8333

Catalog Number Size Price
LS-B8333-50 50 µg (0.5 mg/ml) $425 
Anti-CRD-BP / IGF2BP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IGF2BP1 antibody (0.3 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-CRD-BP / IGF2BP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IGF2BP1 antibody (0.3 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ CRD‑BP / ZBP1 / IGF2BP1 Antibody aa19‑32 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8333

Note: This antibody replaces LS-C112987
Antibody:
Goat Polyclonal to Human CRD-BP / ZBP1 / IGF2BP1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8333-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CRD-BP / ZBP1 / IGF2BP1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
IGF2BP1 antibody LS-B8333 is an unconjugated goat polyclonal antibody to IGF2BP1 (CRD-BP / ZBP1 ) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CRD-BP / ZBP1 / IGF2BP1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-EKVFAEHKISYSGQ, from the internal region of the protein sequence according to NP_006537.3NP_001153895.1.
Blocking Peptide
LS-E27378 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8333. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa19-32
Specificity
Human IGF2BP1. This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3;). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRD-BP / ZBP1 / IGF2BP1
Q9NZI8 NM_006546 NP_006537.3

Validation

Anti-CRD-BP / IGF2BP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC Validated
Anti-CRD-BP / IGF2BP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-CRD-BP / IGF2BP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Anti-IGF2BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
IGF2BP1 antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: Western blot
Immunohistochemistry of paraffin-embedded human stomach using IGF2BP1 antibodyat dilution of 1:100 (40x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation
Product
Species: Human
Applications: Flow Cytometry
Reactivity: Rat
Range: 3.12-200 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 8/22/2019
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