Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B16942-50 50 µl (1 mg/ml) $460 
CFL1 / Cofilin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CFL1 / Cofilin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
CFL1 / Cofilin Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
CFL1 / Cofilin Antibody - Immunofluorescent analysis of Cofilin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CFL1 / Cofilin Antibody - Western blot analysis of Cofilin expression in Jurkat (A); HeLa (B); MCF7 (C); A431 (D) whole cell lysates.
CFL1 / Cofilin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CFL1 / Cofilin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
CFL1 / Cofilin Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
CFL1 / Cofilin Antibody - Immunofluorescent analysis of Cofilin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CFL1 / Cofilin Antibody - Western blot analysis of Cofilin expression in Jurkat (A); HeLa (B); MCF7 (C); A431 (D) whole cell lysates.
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

IHC‑plus™ Polyclonal Rabbit anti‑Human CFL1 / Cofilin Antibody (N‑Terminus, IHC, IF, WB) LS‑B16942

IHC‑plus™ Polyclonal Rabbit anti‑Human CFL1 / Cofilin Antibody (N‑Terminus, IHC, IF, WB) LS‑B16942

Note: This antibody replaces LS-C351991
Antibody:
CFL1 / Cofilin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16942-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CFL1 / Cofilin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Cofilin antibody LS-B16942 is an unconjugated rabbit polyclonal antibody to Cofilin (CFL1) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CFL1 / Cofilin
Synonyms
CFL1 | 18 kDa phosphoprotein | Cofilin | CFL | Cofilin 1 (non-muscle) | Cofilin, non-muscle isoform | p18
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
This antibody is purified by immunogen affinity chromatography
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Cofilin.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of Cofilin protein.
Applications
  • IHC - Paraffin (1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFL1 / Cofilin
P23528 NM_005507 NP_005498.1

Validation

CFL1 / Cofilin Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

CFL1 / Cofilin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

CFL1 / Cofilin Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CFL1 / Cofilin Antibody (N-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B16942 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024