Human CENPF / CENP-F

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

Unconjugated

Protein G purified

Unmodified

aa1759-2093

Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen (10 µg/ml)
• Western blot (1 - 10 µg/ml)
• Immunoprecipitation (1 - 10 µg/ml)

Immunohistochemistry: LS-B3157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3157 was determined to be 10 ug/ml. Western Blot: 1-10 ug/ml. Immunoprecipitation: 1-10 ug/ml. Immunohistochemistry: 10 ug/ml with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at pH 6.0.

PBS, pH 7.4

Store at -70°C for up to 1 year. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P49454 NCBI: NM_016343 NP_057427.3