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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3046-50 | 50 µl (1 mg/ml) | $440 |
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2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human CENPF / CENP‑F Antibody (clone 14C10 1D8, aa1759‑2093, IHC, IF, WB) LS‑B3046
IHC‑plus™ Monoclonal Mouse anti‑Human CENPF / CENP‑F Antibody (clone 14C10 1D8, aa1759‑2093, IHC, IF, WB) LS‑B3046
Note: This antibody replaces LS-C19955
Antibody:
CENPF / CENP-F Mouse anti-Human Monoclonal (aa1759-2093) (14C10 1D8) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CENPF / CENP-F Mouse anti-Human Monoclonal (aa1759-2093) (14C10 1D8) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CENP-F antibody LS-B3046 is an unconjugated mouse monoclonal antibody to human CENP-F (CENPF) (aa1759-2093). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human CENPF / CENP-F
Synonyms
CENPF | AH antigen | Centromere protein F | CENF | CENP-F | Kinetochore protein CENPF | Mitosin | CENP-F kinetochore protein | Hcp-1 | PRO1779
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
14C10 1D8
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
Epitope
aa1759-2093
Specificity
Reacts with human Mitosin.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Immunofluorescence
- Western blot (1:500)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3046 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3046 was determined to be 10 ug/ml.
Presentation
PBS
Storage
Keep as concentrated solution. For short term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CENPF / CENP-F
Validation
Anti-CENPF antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CENPF antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CENPF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CENPF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CENPF / CENP-F Antibody (aa1759-2093, clone 14C10 1D8) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B3046 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, Rothkamm K, Mansour WY. Oncotarget. 2016 7:57679-57693. (IF; Human)
Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Mansour WY, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube-Magg C, Borgmann K, Simon R, Petersen C, Dikomey E, Rothkamm K. Scientific reports. 2018 March;8:3947. (Human)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Reactivity:
Mouse
Range:
2.47-200 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
