Target
Mouse IgG1 + IgG2a + IgG2b + IgG3 Fc
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
FITC,
HRP.
Immunogen
Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse IgG, Fc specific.
Usage
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG. as secondary antibody to precipitate the immunoglobulin in normal mouse serum. to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.
Presentation
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee