Mouse IgG1 + IgG2a + IgG2b + IgG3 Fc

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP.

Purified IgG

Unmodified

Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG, Fc specific.

• Immunofluorescence
• ELISA
• Dot Blot

The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various anti- serum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non- isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.