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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IGFBP5 Antibody (Biotin) LS‑C104305
Note: This antibody replaces LS-C36905
IGFBP5 antibody LS-C104305 is a biotin-conjugated rabbit polyclonal antibody to human IGFBP5. Validated for ELISA and WB.
Catalog
Size
Price
LS-C104305-25
25 µg
$285
LS-C104305-50
50 µg
$325
Description
IGFBP5 antibody LS-C104305 is a biotin-conjugated rabbit polyclonal antibody to human IGFBP5. Validated for ELISA and WB.
Target
Human IGFBP5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.25 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
IGFBP5 antibody was raised against e.coli derived recombinant Human IGF-BP5.
Specificity
Human IGF-BP5
Usage
ELISA: To detect hIGF-BP5 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. To detect hIGF-BP5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-BP5 (LS-B8207) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About IGFBP5
P24593 NM_000599 NP_000590.1

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Images

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced
Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Reduced
Biotinylated Anti-Human IGF-BP5 Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human IGF-BP5 Sandwich ELISA
Biotinylated Anti-Human IGF-BP5 Sandwich ELISA

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced
Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Reduced
Biotinylated Anti-Human IGF-BP5 Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human IGF-BP5 Sandwich ELISA
Biotinylated Anti-Human IGF-BP5 Sandwich ELISA

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced
Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Reduced
Biotinylated Anti-Human IGF-BP5 Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human IGF-BP5 Sandwich ELISA
Biotinylated Anti-Human IGF-BP5 Sandwich ELISA

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced
Biotinylated Anti-Human IGF-BP5 Western Blot Unreduced

Western blot

Biotinylated Anti-Human IGF-BP5 Western Blot Reduced
Biotinylated Anti-Human IGF-BP5 Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Biotinylated Anti-Human IGF-BP5 Sandwich ELISA
Biotinylated Anti-Human IGF-BP5 Sandwich ELISA

Requested From: United States
Date Requested: 12/16/2018
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