(tested or 100% immunogen sequence identity)
- IHC (15 µg/ml)
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
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IGFBP5 antibody was raised against purified, NS0-derived, recombinant human insulin-like growth factor binding protein 5.
Recognizes human IGFBP5. Recognizes rhIGFBP-5 in direct ELISAs and western blots. In Direct ELISA and Western Blot. Shows approximately 25% cross-reactivity with rmIGFBP-5 and less than 5% cross-reactivity with rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4,
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Western blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human IGFBP-5. The detection limit for rhIGFBP-5 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 ug/ml. Detects IGFBP-5 in cells and tissues. Direct ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human IGFBP-5. The detection limit for rhIGFBP-5 is approximately 0.2 ng/well.
Lyophilized from a 0.2 um filtered solution in PBS, 5% trehalose. Reconstitution: sterile PBS. If 1 ml of PBS is used, the antibody concentration will be 0.1 mg/ml
1,000 µl Sterile 40-50% glycerol
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.