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Antibodies > IGFBP2 / IGF-BP53 > LS-C756478
 
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Catalog Number Size Price
LS-C756478-10 10 µg $318 
LS-C756478-100 100 µg $470 
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-IGFBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-IGFBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-IGFBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IGFBP2 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IGFBP2 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - Western blot analysis of IGFBP2 using anti-IGFBP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse Neuro-2a whole cell lysates,Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 40KD. The expected band size for IGFBP2 is at 35KD.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-IGFBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-IGFBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-IGFBP2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IGFBP2 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IGFBP2 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody - Western blot analysis of IGFBP2 using anti-IGFBP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse Neuro-2a whole cell lysates,Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 40KD. The expected band size for IGFBP2 is at 35KD.
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Polyclonal Rabbit anti‑Rat IGFBP2 / IGF‑BP53 Antibody (IHC, WB) LS‑C756478

Polyclonal Rabbit anti‑Rat IGFBP2 / IGF‑BP53 Antibody (IHC, WB) LS‑C756478

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Antibody:
IGFBP2 / IGF-BP53 Rabbit anti-Rat Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756478-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IGFBP2 / IGF-BP53 Rabbit anti-Rat Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IGF-BP53 antibody LS-C756478 is an unconjugated rabbit polyclonal antibody to IGF-BP53 (IGFBP2) from rat. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
Target
Rat IGFBP2 / IGF-BP53
Synonyms
IGFBP2 | BP2 | IGFBP-2 | IGF-binding protein 2 | IBP-2 | IBP2 | IGF-BP53
Host
Rabbit
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rat IGFBP2 recombinant protein (Position: E35-Q304).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IGFBP2 / IGF-BP53
P18065 NM_000597 NP_000588.2

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Applications: Western blot, Immunoprecipitation, ELISA
IGFBP2 / IGF-BP53 Antibody - IHC of paraffin-embedded human lung cancer (A), recturn(B), prostate (C), colon cancer (D) showing cytoplasmic localization using IGFBP2 mouse monoclonal antibody with DAB staining.
IGFBP2 / IGF‑BP53 Antibody (clone 1F6F6) LS‑C108883
Species: Human
Applications: IHC, Western blot, ELISA
IGFBP2 / IGF-BP53 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP2.
IGFBP2 / IGF‑BP53 Antibody (clone OTI1A7) LS‑C173803
Species: Human
Applications: Western blot, Flow Cytometry
IGFBP2 / IGF-BP53 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
IGFBP2 / IGF‑BP53 Antibody (aa215‑264) LS‑B11951
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
See all IGFBP2 / IGF-BP53 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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