LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
IGF2R / CD222 Antibody LS‑C497623
CD222 antibody LS-C497623 is an unconjugated rabbit monoclonal antibody to CD222 (IGF2R) from human, mouse and rat. Validated for Flow, IF, IHC, IP and WB.
Catalog
Size
Price
LS-C497623-50
50 µl
$295
LS-C497623-100
100 µl
$385
LS-C497623-200
200 µl
$560

Popular IGF2R / CD222 Products

Anti-IGF2R antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5653 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Non-Human Primates
Applications: Western blot, Flow Cytometry
Immunohistochemical analysis of CD222 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Bovine
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, ELISA
Permeabilized Peripheral Human Granulocytes stained with Mouse anti Human CD222: APC. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rhesus monkey
Applications: Flow Cytometry

Product Description

IGF2R / CD222 Antibody for IHC, IF/Immunofluorescence, WB/Western, IP, Flow LS-C497623

Specifications

Target
Human IGF2R / CD222
Synonyms
IGF2R, CD222 antigen, CIMPR, CI Man-6-P receptor, CI-MPR, IGF-II receptor, MPR 300, MPR1, MPRI, M6P-R, M6P/IGF2R, CD222, M6P/IGF2 receptor
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:20 - 1:50)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IGF2R / CD222 antibody was raised against recombinant protein of human IGF2R
Usage
The predicted MW is 274kDa, while the observed MW by Western blot was 274kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Restrictions
For research use only.
About IGF2R / CD222
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. P11717 NM_000876 NP_000867.2


Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HepG2 cells using IGF2R antibody.
Immunofluorescence analysis of HepG2 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.
Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of HeLa cells using IGF2R antibody.
Immunofluorescence analysis of HeLa cells using IGF2R antibody.

Western blot

Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HepG2 cells using IGF2R antibody.
Immunofluorescence analysis of HepG2 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.
Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of HeLa cells using IGF2R antibody.
Immunofluorescence analysis of HeLa cells using IGF2R antibody.

Western blot

Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HepG2 cells using IGF2R antibody.
Immunofluorescence analysis of HepG2 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.
Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of HeLa cells using IGF2R antibody.
Immunofluorescence analysis of HeLa cells using IGF2R antibody.

Western blot

Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using IGF2R antibodyat dilution of 1:100 (40x lens).

Immunofluorescence

Immunofluorescence analysis of HepG2 cells using IGF2R antibody.
Immunofluorescence analysis of HepG2 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.
Immunofluorescence analysis of MCF-7 cells using IGF2R antibody.

Immunofluorescence

Immunofluorescence analysis of HeLa cells using IGF2R antibody.
Immunofluorescence analysis of HeLa cells using IGF2R antibody.

Western blot

Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using IGF2R antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Requested From: United States
Date Requested: 10/19/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy