Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/ml), a concentration of 3.1-4.6 ug/ml of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.