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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IGF2 Antibody IHC-plus™ LS-B7415

Best Seller
Note: This antibody replaces LS-C104357, LS-C42967
IGF2 antibody LS-B7415 is an unconjugated rabbit polyclonal antibody to human IGF2. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B7415-50
50 µg
$425
Description
IGF2 antibody LS-B7415 is an unconjugated rabbit polyclonal antibody to human IGF2. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human IGF2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IGF2 antibody was raised against e.coli derived recombinant Human IGF-II.
Specificity
Human IGF-II
Usage
Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/ml), a concentration of 3.1-4.6 ug/ml of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About IGF2
P01344 NM_000612 NP_000603.1

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Publications (1)

1
The stem cell transcription factor ZFP57 induces IGF2 expression to promote anchorage-independent growth in cancer cells. Tada Y, Yamaguchi Y, Kinjo T, Song X, Akagi T, Takamura H, Ohta T, Yokota T, Koide H. Oncogene. 2014 Feb;34:752-60. [Full Text Article] [PubMed:24469060]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (reducing) of IGF-II / IGF2 antibody
Western Blot (reducing) of IGF-II / IGF2 antibody

Western blot

Western Blot (non-reducing) of IGF-II / IGF2 antibody
Western Blot (non-reducing) of IGF-II / IGF2 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IGF-II / IGF2 antibody
Sandwich ELISA of IGF-II / IGF2 antibody

Immunohistochemistry

Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (reducing) of IGF-II / IGF2 antibody
Western Blot (reducing) of IGF-II / IGF2 antibody

Western blot

Western Blot (non-reducing) of IGF-II / IGF2 antibody
Western Blot (non-reducing) of IGF-II / IGF2 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IGF-II / IGF2 antibody
Sandwich ELISA of IGF-II / IGF2 antibody

Immunohistochemistry

Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (reducing) of IGF-II / IGF2 antibody
Western Blot (reducing) of IGF-II / IGF2 antibody

Western blot

Western Blot (non-reducing) of IGF-II / IGF2 antibody
Western Blot (non-reducing) of IGF-II / IGF2 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IGF-II / IGF2 antibody
Sandwich ELISA of IGF-II / IGF2 antibody

Immunohistochemistry

Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (reducing) of IGF-II / IGF2 antibody
Western Blot (reducing) of IGF-II / IGF2 antibody

Western blot

Western Blot (non-reducing) of IGF-II / IGF2 antibody
Western Blot (non-reducing) of IGF-II / IGF2 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IGF-II / IGF2 antibody
Sandwich ELISA of IGF-II / IGF2 antibody

Requested From: United States
Date Requested: 2/22/2019
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