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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IGF2 Antibody (aa39‑68) LS‑C165143
IGF2 antibody LS-C165143 is an unconjugated rabbit polyclonal antibody to IGF2 from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C165143-400
400 µl
$305

Popular IGF2 Products

Anti-IGF2 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-IGF-II / IGF2 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: IHC

Product Description

IGF2 antibody LS-C165143 is an unconjugated rabbit polyclonal antibody to IGF2 from human and mouse. Validated for Flow, IHC and WB.

Specifications

Target
Human IGF2
Synonyms
IGF2, C11orf43, Insulin-like growth factor II, IGF-II, PP9974, Somatomedin-A
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E844 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C165143. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa39-68
Specificity
This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About IGF2
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. P01344 NM_000612 NP_000603.1


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Images

Immunohistochemistry

IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Western blot

IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Flow Cytometry

IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Western blot

IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Flow Cytometry

IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Western blot

IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Flow Cytometry

IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IGF2 Antibody (Center R54) immunohistochemistry of formalin-fixed and paraffin-embedded human placenta tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of mouse cerebellum tissue lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Western blot

IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).
IGF2 Antibody (Center R54) western blot of HeLa cell line lysates (35 ug/lane). The IGF2 antibody detected the IGF2 protein (arrow).

Flow Cytometry

IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IGF2 Antibody (Center R54) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/16/2018

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