(tested or 100% immunogen sequence identity)
NI 69 (A89-034) and NI 184 (A89-035)
Also available Unconjugated
or conjugated with FITC
- IHC - Paraffin
- Dot Blot
- Applications tested for the base form of this product only
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Highly purified monoclonal IgA isolated from human serum.
Human IgA, isotype specific.
The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in hemagglutination, hemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:250 upwards. in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitute with distilled water.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.