Human IgA1,2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NI 69 (A89-034) and NI 184 (A89-035)

Horseradish Peroxidase. Also available Unconjugated or conjugated with FITC, TRITC, Biotin.

Purified IgG

Unmodified

Highly purified monoclonal IgA isolated from human serum.

Human IgA, isotype specific.

• IHC
• IHC - Paraffin
• ICC
• ELISA
• Dot Blot
• Applications tested for the base form of this product only

The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in hemagglutination, hemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50. in ELISA from 1:50 upwards. in Western blotting from 1:100 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.