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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSD11B1 / HSD11B Antibody (aa232‑292) IHC‑plus™ LS‑B10496
Note: This antibody replaces LS-C185636
HSD11B antibody LS-B10496 is an unconjugated rabbit polyclonal antibody to HSD11B (HSD11B1) from human and sheep. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10496-50
50 µl (1 mg/ml)
Unavailable

Popular HSD11B1 / HSD11B Products

Anti-HSD11B1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1597 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, Western blot
Antibody (0.3 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
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Species: Human, Mouse, Rat
Applications: Immunoprecipitation

Product Description

HSD11B antibody LS-B10496 is an unconjugated rabbit polyclonal antibody to HSD11B (HSD11B1) from human and sheep. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About HSD11B1 / HSD11B
HSD11B1 / HSD11B is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. P28845 NM_005525 NP_005516.1

HSD11B1 Antibody, 11-beta-HSD1 Antibody, 11-DH Antibody, CORTRD2 Antibody, HSD11B Antibody, HSD11L Antibody, SDR26C1 Antibody, HDL Antibody, HSD11 Antibody


Specifications

Target
Human HSD11B1 / HSD11B
Host
Rabbit
Reactivity
Human, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to a region within amino acids 232 and 292 of HSD11B1.
Epitope
aa232-292
Specificity
Human HSD11B1
Presentation
PBS, pH 7.0, 40% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.

Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.

Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.

Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HSD11B1 / HSD11B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/22/2018

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