Histone antibody was raised against the immunogen prepared was intact calf histone core complexed with rRNA. Calf histone core has high homology to many other mammalian histone core proteins.
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Histones are highly alkaline proteins found in eukaryotic cell nuclei that package and order the DNA into structural units called nucleosomes. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation. Without histones, the unwound DNA in chromosomes would be very long (a length to width ratio of more than 10 million to 1 in human DNA).