(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- ICC (1 - 5 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Histone antibody was raised against purified nuclear fractions of HeLa cells.
Binds specifically to an antigen determinant, which is present on all five histone proteins. Recognizes the histones H1, H2A, H2B, H3 and H4 with comparable sensitivity. Species cross-reactivity: Human, bovine, rat, Xenopus, opossum and hamster.
0.01 M PBS, pH 7.6, 0.09% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
Histones are highly alkaline proteins found in eukaryotic cell nuclei that package and order the DNA into structural units called nucleosomes. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation. Without histones, the unwound DNA in chromosomes would be very long (a length to width ratio of more than 10 million to 1 in human DNA).