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Catalog Number Size Price
LS-C676784-50 50 µl $298 
LS-C676784-100 100 µl $370 
HIST1H4I Antibody - Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) diluted at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H4I Antibody - Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H4A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
HIST1H4I Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate(treated with 30mM sodium butyrate for 4h or not) All Lanes: HIST1H4A antibody at 1.1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 KDa Observed band size: 12 KDa
HIST1H4I Antibody - Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) Lane 1: Rabbit control IgG instead of 2-hydroxyisobutyryl-HIST1H4A (K16) Antibody in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h).For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: 2-hydroxyisobutyryl-HIST1H4A (K16) Antibody (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) 500µg) Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
HIST1H4I Antibody - Chromatin Immunoprecipitation Hela (4*10E6, treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (2-hydroxyisobutyryl-HIST1H4A (K16) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
HIST1H4I Antibody - Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) diluted at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H4I Antibody - Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H4A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
HIST1H4I Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate(treated with 30mM sodium butyrate for 4h or not) All Lanes: HIST1H4A antibody at 1.1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 KDa Observed band size: 12 KDa
HIST1H4I Antibody - Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) Lane 1: Rabbit control IgG instead of 2-hydroxyisobutyryl-HIST1H4A (K16) Antibody in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h).For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: 2-hydroxyisobutyryl-HIST1H4A (K16) Antibody (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) 500µg) Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
HIST1H4I Antibody - Chromatin Immunoprecipitation Hela (4*10E6, treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (2-hydroxyisobutyryl-HIST1H4A (K16) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
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Polyclonal Rabbit anti‑Human HIST1H4I Antibody (IF, WB) LS‑C676784

Polyclonal Rabbit anti‑Human HIST1H4I Antibody (IF, WB) LS‑C676784

Antibody:
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$298
LS-C676784-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HIST1H4I Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H4I antibody LS-C676784 is an unconjugated rabbit polyclonal antibody to human HIST1H4I. Validated for ChrIP, ELISA, IF and WB.
Target
Human HIST1H4I
Synonyms
HIST1H4I | HIST2H4 | Histone 1, H4i | Histone cluster 1, H4i | Histone family member | Histone H4 | Histone 4 family, member M
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Peptide sequence around site of 2-hydroxyisobutyryl-Lys (16) derived from human Histone H4
Applications
  • Immunofluorescence
  • Western blot
  • ELISA
  • Chromatin Immunoprecipitation
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H4I
P62805 NM_003495 NP_003486.1

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Requested From: United States
Date Requested: 4/28/2024