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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C153765-100 100 µl (38 mg/ml) $425 
HAUS8 Antibody - Anti-HICE1 in Western blot of Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

Polyclonal Rabbit anti‑Human HAUS8 Antibody (WB) LS‑C153765

Polyclonal Rabbit anti‑Human HAUS8 Antibody (WB) LS‑C153765

Antibody:
Rabbit Polyclonal to Human HAUS8
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153765-100
100 µl
38 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HAUS8
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HAUS8 antibody LS-C153765 is an unconjugated rabbit polyclonal antibody to human HAUS8. Validated for ELISA and WB.
Target
Human HAUS8
Synonyms
HAUS8 | DGT4 | NY-SAR-48 | Sarcoma antigen NY-SAR-48 | HICE1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Orangutan (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Applications
  • Western blot (1:10000)
  • ELISA (1:250000)
Immunogen
Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
Specificity
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.
Usage
This antiserum has been tested for use in ELISA and western blotting using a full length recombinant Hice1 protein. Specific conditions for reactivity and detection of Hice1 should be optimized by the end user. Expect a band approximately ~45 kD in size corresponding to Hice1 by Western Blot in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HAUS8
Q9BT25 Q9BT25

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Requested From: United States
Date Requested: 6/3/2020