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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HAUS8 Antibody LS‑C153765

Catalog Number Size Price
LS-C153765-100 100 µl (38 mg/ml) $425 
Anti-HICE1 in Western blot of Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

HAUS8 Antibody LS‑C153765

Antibody:
Rabbit Polyclonal to Human HAUS8
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153765-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HAUS8
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HAUS8 antibody LS-C153765 is an unconjugated rabbit polyclonal antibody to human HAUS8. Validated for ELISA and WB.
Target
Human HAUS8
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:10000)
  • ELISA
Immunogen
Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
Specificity
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.
Usage
This antiserum has been tested for use in ELISA and western blotting using a full length recombinant Hice1 protein. Specific conditions for reactivity and detection of Hice1 should be optimized by the end user. Expect a band approximately ~45 kD in size corresponding to Hice1 by Western Blot in the appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HAUS8
Q9BT25 Q9BT25

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Featured Products

Western blot of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 pS70 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70. Other band(s): none.
Species: Human
Applications: Western blot, ELISA
IHC of paraffin-embedded Human lymph tissue using HAUS8 antibody at 1:50 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Reactivity: Human, Mouse, Rat
Range: 12.35-1000 pg/ml
Reactivity: Human
Range: 78.125-5000 pg/ml

Requested From: United States
Date Requested: 8/23/2019
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