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Catalog Number Size Price
LS-B10617-50 50 µg (1 mg/ml) $485 
HAUS8 Antibody - Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HAUS8 Antibody - Western Blot of Rabbit anti-HAUS8 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 µl per lane. Primary antibody: HAUS8 antibody at 1.24 ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HAUS8. Other band(s): HAUS8 splice variants and isoforms.
HAUS8 Antibody - Western blot of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.
HAUS8 Antibody - Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HAUS8 Antibody - Western Blot of Rabbit anti-HAUS8 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 µl per lane. Primary antibody: HAUS8 antibody at 1.24 ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HAUS8. Other band(s): HAUS8 splice variants and isoforms.
HAUS8 Antibody - Western blot of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.
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IHC‑plus™ Polyclonal Rabbit anti‑Human HAUS8 Antibody (IHC, WB) LS‑B10617

IHC‑plus™ Polyclonal Rabbit anti‑Human HAUS8 Antibody (IHC, WB) LS‑B10617

Note: This antibody replaces LS-C153859
Antibody:
HAUS8 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B10617-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HAUS8 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HAUS8 antibody LS-B10617 is an unconjugated rabbit polyclonal antibody to human HAUS8. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HAUS8
Synonyms
HAUS8 | DGT4 | NY-SAR-48 | Sarcoma antigen NY-SAR-48 | HICE1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.
Specificity
A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.45 µg/ml)
  • ELISA (1:30000 - 1:45000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kD in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HAUS8
Q9BT25 Q9BT25

Validation

HAUS8 Antibody - Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ HAUS8 Antibody for IHC, WB/Western, ELISA LS-B10617 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024