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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Gravin / AKAP12 Antibody (aa1719‑1782) IHC‑plus™ LS‑B10829
Note: This antibody replaces LS-C185800
AKAP12 antibody LS-B10829 is an unconjugated rabbit polyclonal antibody to human AKAP12 (Gravin). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10829-100
100 µl (0.73 mg/ml)
$425
Description
AKAP12 antibody LS-B10829 is an unconjugated rabbit polyclonal antibody to human AKAP12 (Gravin). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Gravin / AKAP12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (7.5 µg/ml)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Gravin / AKAP12 antibody was raised against synthetic peptide corresponding to a region within amino acids 1719 and 1782 of AKAP12 (SwissProt Q02952).
Epitope
aa1719-1782
Specificity
Human Gravin / AKAP12
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About Gravin / AKAP12
Q02952 NM_005100 NP_005091.2

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Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/ 5%NFDM, probed with 5 g/ml of pre-immune serum 5 ug of SSeCKS – myristylated (PKC) substrate antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP 1:10,000, and detected with luminol.
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Images

Immunohistochemistry

Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.

Western blot

Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000
Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000

Immunohistochemistry

Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.

Western blot

Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000
Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000

Immunohistochemistry

Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.

Western blot

Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000
Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000

Immunohistochemistry

Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using AKAP12 antibody at 1:200 dilution.

Western blot

Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000
Sample (30g whole cell lysate). A: HeLa S3. 5% SDS PAGE. Gravin / AKAP12 antibody diluted at 1:1000

Requested From: United States
Date Requested: 11/17/2018
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