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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Gravin / AKAP12 Antibody (aa1645‑1658) LS‑C139806
AKAP12 antibody LS-C139806 is an unconjugated goat polyclonal antibody to AKAP12 (Gravin) from mouse and rat. Validated for Peptide-ELISA.
Antibody
Catalog
Size
Price
LS-C139806-100
100 µg
$335

Popular Gravin / AKAP12 Products

Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/ 5%NFDM, probed with 5 g/ml of pre-immune serum 5 ug of SSeCKS – myristylated (PKC) substrate antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP 1:10,000, and detected with luminol.
Species: Rat, Bovine
Applications: IHC, Western blot, Immunoprecipitation
Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP12 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, ELISA
Immunohistochemistry of Human ovarian cancer using AKAP12 Polyclonal Antibody at dilution of 1:40.
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

AKAP12 antibody LS-C139806 is an unconjugated goat polyclonal antibody to AKAP12 (Gravin) from mouse and rat. Validated for Peptide-ELISA.

Specifications

Target
Mouse Gravin / AKAP12
Synonyms
AKAP12, A-kinase anchor protein 12, AKAP-12, AKAP250, AKAP 250, Gravin, Kinase scaffold protein gravin, Myasthenia gravis autoantigen, SSeCKS
Host
Goat
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Immunogen
Gravin / AKAP12 antibody was raised against synthetic peptide C-SPDTNGPKLTEEGD from an internal region of mouse AKAP12 (NP_112462.1). Percent identity by BLAST analysis: Mouse, Rat (100%).
Blocking Peptide
LS-E27711 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139806. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1645-1658
Specificity
Mouse AKAP12. This antibody is expected to recognize isoform 1 (NP_005091.2) only.
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments in lysates of cell lines NIH3T3 and of Mouse and Rat Brain and Testis gave no specific signal but low background (at antibody concentration up to 2 ug/ml).
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About Gravin / AKAP12
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. Q02952 NM_005100 NP_005091.2


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Requested From: United States
Date Requested: 11/14/2018

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