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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Gravin / AKAP12 Antibody (aa1485‑1782, Biotin) LS‑C688445
AKAP12 antibody LS-C688445 is a biotin-conjugated rabbit polyclonal antibody to human AKAP12 (Gravin). Validated for IHC and WB.
Catalog
Size
Price
LS-C688445-20
20 µl
$205
LS-C688445-100
100 µl
$265
LS-C688445-200
200 µl
$315
LS-C688445-1
1 ml
$575
LS-C688445-10
10 ml
$2,310

Popular Gravin / AKAP12 Products

Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/ 5%NFDM, probed with 5 g/ml of pre-immune serum 5 ug of SSeCKS – myristylated (PKC) substrate antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP 1:10,000, and detected with luminol.
Species: Rat, Bovine
Applications: IHC, Western blot, Immunoprecipitation
Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP12 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, ELISA
Immunohistochemistry of Human ovarian cancer using AKAP12 Polyclonal Antibody at dilution of 1:40.
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

AKAP12 antibody LS-C688445 is a biotin-conjugated rabbit polyclonal antibody to human AKAP12 (Gravin). Validated for IHC and WB.

Specifications

Target
Human Gravin / AKAP12
Synonyms
AKAP12, A-kinase anchor protein 12, AKAP-12, AKAP250, AKAP 250, Gravin, Kinase scaffold protein gravin, Myasthenia gravis autoantigen, SSeCKS
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, Biotin, FITC, Cy3, HRP, PE, APC, Cy7, APC.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Gravin / AKAP12 antibody was raised against recombinant AKAP12 (Pro1485-Ser1782)
Epitope
aa1485-1782
Specificity
The antibody is a rabbit polyclonal antibody raised against AKAP12. It has been selected for its ability to recognize AKAP12 in immunohistochemical staining and western blotting.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About Gravin / AKAP12
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. Q02952 NM_005100 NP_005091.2


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Images

Western blot

Western blot of Gravin / AKAP12 antibody.
Western blot of Gravin / AKAP12 antibody.

Western blot

Western blot of Gravin / AKAP12 antibody.
Western blot of Gravin / AKAP12 antibody.

Western blot

Western blot of Gravin / AKAP12 antibody.
Western blot of Gravin / AKAP12 antibody.

Western blot

Western blot of Gravin / AKAP12 antibody.
Western blot of Gravin / AKAP12 antibody.

Requested From: United States
Date Requested: 11/12/2018

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