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Catalog Number Size Price
LS-C69829-1 1 mg $640 
Antibody

Polyclonal Goat Anti‑Human IgG Fab'2 Secondary Antibody (AP, IHC, WB) LS‑C69829

Polyclonal Goat Anti‑Human IgG Fab'2 Secondary Antibody (AP, IHC, WB) LS‑C69829

Antibody:
Human IgG Fab'2 Goat anti-Human Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified
Price
Catalog Number
$640
LS-C69829-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Human IgG Fab'2 Goat anti-Human Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified

Specifications

Description
Human IgG Fab'2 antibody LS-C69829 is an AP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Human IgG Fab'2
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Human IgG F (ab')2 fragment.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG, human IgG F (ab')2 and human serum. No reaction was observed against human IgG F(c).
Applications
  • IHC
  • Western blot
  • ELISA (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (Western or Dot Blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilution (s): ELISA: 1:2000 to 1:9000. Assayed against 1 ug of human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT. Optimal titers for other applications should be determined by the researcher. Storage Conditions: Store vial at 4? C before opening. DO NOT FREEZE. This product is stable at 4? C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one year from date of opening product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/19/2024