Human IgG Fab'2

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Alkaline Phosphatase

Immunoaffinity purified

Unmodified

Human IgG F (ab')2 fragment.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG, human IgG F (ab')2 and human serum. No reaction was observed against human IgG F(c).

• IHC
• Western blot
• ELISA (1:2000)

Suitable for immunoblotting (Western or Dot Blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilution (s): ELISA: 1:2000 to 1:9000. Assayed against 1 ug of human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT. Optimal titers for other applications should be determined by the researcher. Storage Conditions: Store vial at 4? C before opening. DO NOT FREEZE. This product is stable at 4? C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one year from date of opening product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.

Store at 4°C. Do not freeze.

For research use only. Intended for use by laboratory professionals.

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