Human IgA + IgG + IgM

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified

Unmodified

Highly purified IgG, and pools of homogenous IgA, IgM and IgD isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Human immunoglobulins IgG, IgA, IgM and IgD, heavy and light chains.

• IHC
• ICC
• Immunofluorescence
• ELISA

The defined antibody specificity is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibody to common determinants and to class specific determinants and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA, IgM and IgD (if present in precipitable amounts) precipitin lines are obtained. This antiserum is intended to detect and identify polyclonal immunoglobulins, purified monoclonal immunoglobulins in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electro- phoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.