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Catalog Number Size Price
LS-C816418-100 100 µl $412 
GAK Antibody - Immunochemical staining of human GAK in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
GAK Antibody - Immunochemical staining of human GAK in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
GAK Antibody - Immunofluorescence staining of GAK in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human GAK polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
GAK Antibody - Anti-GAK rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 143 kDa.
GAK Antibody - Immunochemical staining of human GAK in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
GAK Antibody - Immunochemical staining of human GAK in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
GAK Antibody - Immunofluorescence staining of GAK in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human GAK polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
GAK Antibody - Anti-GAK rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 143 kDa.
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Polyclonal Rabbit anti‑Human GAK Antibody (IHC, IF, WB) LS‑C816418

Polyclonal Rabbit anti‑Human GAK Antibody (IHC, IF, WB) LS‑C816418

Antibody:
GAK Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C816418-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GAK Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GAK antibody LS-C816418 is an unconjugated rabbit polyclonal antibody to human GAK. Validated for ICC, IF, IHC and WB.
Target
Human GAK
Synonyms
GAK | Auxilin-2 | Cyclin-G-associated kinase | Cyclin G associated kinase | DNAJ26 | DNAJC26
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human Gak fragment
Specificity
Human GAK
Applications
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GAK
O14976 NM_005255 NP_005246.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024