LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
GABRA2 Antibody (aa378‑406) LS‑C166321
GABRA2 antibody LS-C166321 is an unconjugated rabbit polyclonal antibody to human GABRA2. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C166321-400
400 µl
$305

Popular GABRA2 Products

Immunohistochemical analysis of GABRA2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA

Product Description

GABRA2 antibody LS-C166321 is an unconjugated rabbit polyclonal antibody to human GABRA2. Validated for Flow, IHC and WB.

Specifications

Target
Human GABRA2
Synonyms
GABRA2, GABA A receptor alpha 2, GABA(A) receptor, alpha 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E10050 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166321. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa378-406
Specificity
This GABRA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-406 amino acids from the C-terminal region of human GABRA2.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About GABRA2
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. P47869 NM_000807 NP_000798.2


Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).
GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).

Flow Cytometry

GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).
GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).

Flow Cytometry

GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).
GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).

Flow Cytometry

GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GABRA2 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).
GABRA2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GABRA2 antibody detected the GABRA2 protein (arrow).

Flow Cytometry

GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GABRA2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/15/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy