(tested or 100% immunogen sequence identity)
FRS2 antibody was raised against synthetic phosphopeptide corresponding to residues surrounding tyrosine 196 of human FRS2-alpha (KLH coupled).
Detects endogenous levels of human FRS2-alpha only when phosphorylated at tyrosine 196 at 85kD. Does not crossreact with unrelated tyrosine phosphorylated proteins.
Suitable for use in Western Blot and ELISA. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.