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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXC1 Antibody (aa62‑186) LS‑C293970
FOXC1 antibody LS-C293970 is an unconjugated rabbit polyclonal antibody to mouse FOXC1. Validated for IHC and WB.
Catalog
Size
Price
LS-C293970-20
20 µl (0.5 mg/ml)
$195
LS-C293970-100
100 µl (0.5 mg/ml)
$255
LS-C293970-200
200 µl (0.5 mg/ml)
$285
LS-C293970-1
1 ml (0.5 mg/ml)
$485
LS-C293970-10
10 ml (0.5 mg/ml)
$1,935

Popular FOXC1 Products

Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Species: Human, Monkey, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Species: Human, Monkey, Mouse, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Chromatin Immunoprecipitation
Immunofluorescent analysis of Hela cells using FOXC1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

FOXC1 antibody LS-C293970 is an unconjugated rabbit polyclonal antibody to mouse FOXC1. Validated for IHC and WB.

Specifications

Target
Mouse FOXC1
Synonyms
FOXC1, Forkhead-like 7, FREAC3, FKHL7, Forkhead-related activator 3, Forkhead-related protein FKHL7, IGDA, IHG1, IRID1, Forkhead box protein C1, Mesenchyme fork head protein 1, Myeloid factor-delta, ARA, Forkhead box C1, FREAC-3, RIEG3
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, FITC, Cy3, HRP, PE, APC, Cy7, APC.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:100 - 1:400)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXC1 antibody was raised against recombinant FOXC1 (Arg62-Lys186) expressed in E. coli.
Epitope
aa62-186
Specificity
The antibody is a rabbit polyclonal antibody raised against FOXC1. It has been selected for its ability to recognize FOXC1 in immunohistochemical staining and western blotting.
Usage
ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About FOXC1
Forkhead box C1 (FOXC1) belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of FOXC1 has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in FOXC1 cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. Q12948 NM_001453 NP_001444.2


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Images

Western blot

Western blot of recombinant FOXC1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant FOXC1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant FOXC1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant FOXC1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant FOXC1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant FOXC1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant FOXC1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant FOXC1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/14/2018

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