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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXC1 Antibody (aa250‑300) IHC‑plus™ LS‑B1800 Best Seller
Note: This antibody replaces LS-C47366
FOXC1 antibody LS-B1800 is an unconjugated rabbit polyclonal antibody to FOXC1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1800-50
50 µl
$425
Description
FOXC1 antibody LS-B1800 is an unconjugated rabbit polyclonal antibody to FOXC1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FOXC1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:250 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXC1 antibody was raised against a portion of amino acids 250-300of human FOXC1.
Epitope
aa250-300
Specificity
A portion of amino acids 250-300 of human FOXC1.
Usage
Immunohistochemistry: LS-B1800 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1800 was determined to be 1:200.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About FOXC1
Q12948 NM_001453 NP_001444.2

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Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
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Images

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?
Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?
Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?
Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?
Western blot of human FOXC1 in humanuliver lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1:1000 dilution.?

Requested From: United States
Date Requested: 11/18/2018
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