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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXC1 Antibody (aa35‑84, HRP) LS‑C436066
FOXC1 antibody LS-C436066 is an HRP-conjugated rabbit polyclonal antibody to FOXC1 from human, rat, monkey and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C436066-100
100 µl (0.65 mg/ml)
$385
Description
FOXC1 antibody LS-C436066 is an HRP-conjugated rabbit polyclonal antibody to FOXC1 from human, rat, monkey and other species. Validated for IHC and WB.
Target
Human FOXC1
Host
Rabbit
Reactivity
Human, Monkey, Rat, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXC1 antibody was raised against synthetic peptide located between aa35-84 of human FOXC1 (Q12948, NP_001444). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Rat (100%); Elephant (93%); Mouse, Opossum (92%).
Epitope
aa35-84
Specificity
Human FOXC1
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About FOXC1
Q12948 NM_001453 NP_001444.2

Popular FOXC1 Products

Anti-FOXC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Species: Human, Monkey, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Species: Human, Monkey, Mouse, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Chromatin Immunoprecipitation
Immunofluorescent analysis of Hela cells using FOXC1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

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Images

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXC1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXC1 antibody Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/17/2018
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