Human FN1 / Fibronectin

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Unmodified

Purified fibronectin from human plasma.

• IHC - Paraffin (1:500)
• Immunofluorescence (1:80)
• ELISA (1:200)
• Radioimmunoassay

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Human fibronectin 100%, human collagen type I, III, IV and VI <0.1% (RIA at 1:10000 dilution).

PBS

Reconstitute with 0.5 ml sterile water

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02751 NCBI: NM_002026 NP_002017.1