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Catalog Number Size Price
LS-C60153-100 100 µg (1 mg/ml) $559 
FN1 / Fibronectin Antibody - Immunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). Primary antibody: 2 ug/ml for 1 hour @ room T. Secondary antibody: Streptavidin. Conj. HRP 10 ug/ml circa 45 min. @ room T. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti‑Human FN1 / Fibronectin Antibody (Biotin, IHC, WB) LS‑C60153

Polyclonal Rabbit anti‑Human FN1 / Fibronectin Antibody (Biotin, IHC, WB) LS‑C60153

Antibody:
FN1 / Fibronectin Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine
Format:
Biotin, Unmodified
Price
Catalog Number
$559
LS-C60153-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FN1 / Fibronectin Rabbit anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine
Format:
Biotin, Unmodified

Specifications

Description
Fibronectin antibody LS-C60153 is a biotin-conjugated rabbit polyclonal antibody to Fibronectin (FN1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Target
Human FN1 / Fibronectin
Synonyms
FN1 | CIG | Cold-insoluble globulin | Fibronectin 1 | FINC | LETS | GFND | ED-B | Fibronectin | FN | FNZ | GFND2 | Migration-stimulating factor | MSF
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.
Specificity
Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FN1 / Fibronectin
P02751 NM_002026 NP_002017.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024