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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C153834-100 100 µg (1 mg/ml) $425 
ESRP2 / RBM35B Antibody - Anti-ESRP2 by western blot shows detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes ug and 1 contain 5 ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.

Monoclonal Mouse anti‑Mouse ESRP2 / RBM35B Antibody (clone 22C5.H7.A6, WB) LS‑C153834

Monoclonal Mouse anti‑Mouse ESRP2 / RBM35B Antibody (clone 22C5.H7.A6, WB) LS‑C153834

Antibody:
Mouse Monoclonal to Mouse ESRP2 / RBM35B
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153834-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Mouse ESRP2 / RBM35B
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RBM35B antibody LS-C153834 is an unconjugated mouse monoclonal antibody to mouse RBM35B (ESRP2). Validated for ELISA and WB. Cited in 1 publication.
Target
Mouse ESRP2 / RBM35B
Synonyms
ESRP2 | RBM35B | RNA binding motif protein 35A | RNA-binding protein 35B | RNA-binding motif protein 35B | RNA binding motif protein 35B
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
22C5.H7.A6
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA (1:100000)
Immunogen
Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Specificity
This antibody reacts with mouse Esrp-2 protein. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.
Usage
This protein-A purified antibody has been tested for use western blotting and shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kD in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ESRP2 / RBM35B
Q9H6T0 NM_024939 NP_079215.2

LSBio Ratings

ESRP2 / RBM35B Antibody (clone 22C5.H7.A6) for WB/Western, ELISA LS-C153834 has an LSBio Rating of
Publications (4)

Publications (1)

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP. The EMBO journal. 2010 29:3286-300.

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Requested From: United States
Date Requested: 6/5/2020