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ESRP2 / RBM35B Antibody (clone 22C5.H7.A6) LS‑C153834

Catalog Number Size Price
LS-C153834-100 100 µg (1 mg/ml) $425 
Anti-ESRP2 by western blot shows detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes ug and 1 contain 5 ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.

ESRP2 / RBM35B Antibody (clone 22C5.H7.A6) LS‑C153834

Antibody:
Mouse Monoclonal to Mouse ESRP2 / RBM35B
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153834-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Mouse ESRP2 / RBM35B
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RBM35B antibody LS-C153834 is an unconjugated mouse monoclonal antibody to mouse RBM35B (ESRP2 ). Validated for ELISA and WB. Cited in 1 publication.
Target
Mouse ESRP2 / RBM35B
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
22C5.H7.A6
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA
Immunogen
Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Specificity
This antibody reacts with mouse Esrp-2 protein. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.
Usage
This protein-A purified antibody has been tested for use western blotting and shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kD in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ESRP2 / RBM35B
Q9H6T0 NM_024939 NP_079215.2

Publications (1)

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP. The EMBO journal. 2010 29:3286-300.

Reviews (0)

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Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry of RBM35B in human lung tissue with RBM35B antibody at 5 µg/mL.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
Anti-ESRP2 by western blot shows detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes G and 1 contain 5ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.
Species: Mouse
Applications: Western blot, ELISA
ESRP2 / RBM35B antibody Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Xenopus
Applications: Western blot

Requested From: United States
Date Requested: 8/22/2019
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