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Catalog Number Size Price
LS-C154126-100 100 µg (1 mg/ml) $574 
ESRP1 / RBM35A Antibody - Anti-ESRP-1 Antibody - Western Blot. Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

Monoclonal Mouse anti‑Mouse ESRP1 / RBM35A Antibody (clone 27H12.F3.H5, WB) LS‑C154126

Monoclonal Mouse anti‑Mouse ESRP1 / RBM35A Antibody (clone 27H12.F3.H5, WB) LS‑C154126

Antibody:
ESRP1 / RBM35A Mouse anti-Mouse Monoclonal (27H12.F3.H5) Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C154126-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ESRP1 / RBM35A Mouse anti-Mouse Monoclonal (27H12.F3.H5) Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RBM35A antibody LS-C154126 is an unconjugated mouse monoclonal antibody to mouse RBM35A (ESRP1). Validated for ELISA and WB. Cited in 1 publication.
Target
Mouse ESRP1 / RBM35A
Synonyms
ESRP1 | RBM35A | RMB35A | RNA binding motif protein 35A | RNA-binding motif protein 35A | RNA-binding protein 35A
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Rat, Bovine, Dog, Horse, Opossum, Panda, Pig (at least 90% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
27H12.F3.H5
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Specificity
This antibody reacts with mouse Esrp-1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
Applications
  • Western blot (1:1000)
  • ELISA (1:750000)
Usage
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kD in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ESRP1 / RBM35A
Q6NXG1 NM_017697 NP_060167.2

LSBio Ratings

ESRP1 / RBM35A Antibody (clone 27H12.F3.H5) for WB/Western, ELISA LS-C154126 has an LSBio Rating of
Publications (4)

Publications (1)

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP. The EMBO journal. 2010 29:3286-300.

Customer Reviews (0)


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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024