Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > ESR2 / ER Beta > LS-C801216
 
Print Page  
Catalog Number Size Price
LS-C801216-100 100 µl (1 mg/ml) $379 
LS-C801216-200 200 µl (1 mg/ml) $421 
ESR2 / ER Beta Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ESR2 / ER Beta Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ESR2 / ER Beta Antibody - Western blot analysis of Estrogen Receptor-beta phosphorylation expression in HepG2 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ESR2 / ER Beta Antibody - Western blot analysis of Phospho-Estrogen Receptor beta (Ser105) antibody expression in HepG2 cells lysates. The lane on the right is treated with the antigen-specific peptide.
ESR2 / ER Beta Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ESR2 / ER Beta Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ESR2 / ER Beta Antibody - Western blot analysis of Estrogen Receptor-beta phosphorylation expression in HepG2 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ESR2 / ER Beta Antibody - Western blot analysis of Phospho-Estrogen Receptor beta (Ser105) antibody expression in HepG2 cells lysates. The lane on the right is treated with the antigen-specific peptide.
1 of 4
2 of 4
3 of 4
4 of 4

Polyclonal Rabbit anti‑Human ESR2 / ER Beta Antibody (phospho‑Ser105, IF, WB) LS‑C801216

Polyclonal Rabbit anti‑Human ESR2 / ER Beta Antibody (phospho‑Ser105, IF, WB) LS‑C801216

Submit Review LSBio Guarantee
Antibody:
ESR2 / ER Beta Rabbit anti-Human Polyclonal (pSer105) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801216-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ESR2 / ER Beta Rabbit anti-Human Polyclonal (pSer105) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ER Beta antibody LS-C801216 is an unconjugated rabbit polyclonal antibody to ER Beta (ESR2) (pSer105) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human ESR2 / ER Beta
Synonyms
ESR2 | ESTRB | ER Beta | ERbeta | ER-BETA | ESR-BETA | ESRB | Estrogen receptor 2 (ER beta) | NR3A2 | Estrogen receptor beta | Estrogen receptor beta 4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Estrogen Receptor- beta around the phosphorylation site of Serine 105.
Epitope
pSer105
Specificity
Phospho-Estrogen Receptor- beta (Ser105) Antibody detects endogenous levels of Estrogen Receptor- beta only when phosphorylated at Serine 105.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ESR2 / ER Beta
Q92731 NM_001437 NP_001428.1

Publications (0)

Submit a Publication for LS-C801216

Customer Reviews (0)

Submit a review for LS-C801216

Featured Products

ESR2 / ER Beta Antibody - Anti-ER-Beta antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ESR2 / ER Beta Antibody (aa82‑131) LS‑B945
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Product
ESR2 / ER Beta Antibody (aa512‑530) LS‑C15082
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
ESR2 / ER Beta Antibody - Immunofluorescence analysis of HeLa cells, using Estrogen Receptor-beta Antibody. The picture on the right is blocked with the synthesized peptide.
ESR2 / ER Beta Antibody (aa71‑120) LS‑C117893
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ESR2 / ER Beta Antibody - Western blot analysis of Estrogen Receptor beta expression in COLO205 (A); NIH3T3 (B) whole cell lysates.
ESR2 / ER Beta Antibody (Internal) LS‑C356032
Species: Human, Mouse, Rat, Dog
Applications: ICC, Immunofluorescence, Western blot
ESR2 / ER Beta Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ESR2 / ER Beta Antibody (aa94‑118) LS‑C801660
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ESR2 / ER Beta Antibody - ESR2 / ER-Beta antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ESR2 / ER Beta Antibody (aa396‑445) LS‑C109648
Species: Human
Applications: Western blot
See all ESR2 / ER Beta Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com