Human EMA / MUC1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b,k Recombinant Monoclonal

rMUC1/960

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Recombinant full-length human MUC1 protein. Antigen Molecular Weight: 265-400 kDa

This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

• IHC - Paraffin (0.5 - 1 µg/ml)

• IHC - Frozen

Flow Cytometry (0.5-1 ug/million cells) - Immunofluorescence (1-2 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15941 NCBI: NM_002456 NP_002447.4