G9A antibody LS-C15281 is an unconjugated rabbit polyclonal antibody to G9A (EHMT2) from human and monkey. Validated for WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (0.2 - 2 µg/ml)
- (applications tested for the base form of this product only)
EHMT2 / G9A antibody was raised against kLH-conjugated, synthetic peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 17/18 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (94%).
Recognizes human G9a protein, Mr ~140kD. Species cross-reactivity: Human. Predicted to cross-react with mouse based on sequence homology.
Suitable for use in Western Blot. Western Blot: 0.2-2 ug/ml detected G9a in HeLa nuclear extract cell lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-G9a (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.