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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ECM1 Antibody LS‑C335162
ECM1 antibody LS-C335162 is an unconjugated rabbit polyclonal antibody to ECM1 from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C335162-50
50 µl
$305
LS-C335162-200
200 µl
$495

Popular ECM1 Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

ECM1 antibody LS-C335162 is an unconjugated rabbit polyclonal antibody to ECM1 from human, mouse and rat. Validated for IHC and WB.

Specifications

Target
Human ECM1
Synonyms
ECM1, Extracellular matrix protein 1, Secretory component p85
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ECM1 antibody was raised against recombinant protein of human ECM1
Specificity
Human ECM1
Usage
The predicted MW is 19kDa/46kDa/60kDa/63kDa, while the observed MW by Western blot was 55kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ECM1
ECM1 is a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Q16610 NM_004425 NP_004416.2


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Images

Western blot

Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Western blot

Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Western blot

Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Western blot

Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of various cell lines, using ECM1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Requested From: United States
Date Requested: 11/13/2018

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