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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ECM1 Antibody (clone ECM1/792) LS‑C390060
ECM1 antibody LS-C390060 is an unconjugated mouse monoclonal antibody to ECM1 from human and rat. Validated for Flow, IF and IHC.
Catalog
Size
Price
LS-C390060-20
20 µg (0.2 mg/ml)
$255
LS-C390060-100
100 µg (0.2 mg/ml)
$395
Description
ECM1 antibody LS-C390060 is an unconjugated mouse monoclonal antibody to ECM1 from human and rat. Validated for Flow, IF and IHC.
Target
Human ECM1
Host
Mouse
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [ECM1/792]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Ready-to-Use, Azide-free.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ECM1 antibody was raised against recombinant human protein.
Specificity
Human ECM1
Usage
This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression. Optimal dilution of the Secretory Component Glycoprotein antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
About ECM1
Q16610 NM_004425 NP_004416.2

Popular ECM1 Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Secretory Component Glycoprotein antibody (ECM1/792). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/20/2018
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