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Catalog Number Size Price
LS-C801129-100 100 µl (1 mg/ml) $379 
LS-C801129-200 200 µl (1 mg/ml) $421 
E2F1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
E2F1 Antibody - Western blot analysis of extracts of various tissue sample using PDLIM1 antibody.
E2F1 Antibody - Western blot analysis of E2F1 phosphorylation expression in Etoposide treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
E2F1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
E2F1 Antibody - Western blot analysis of extracts of various tissue sample using PDLIM1 antibody.
E2F1 Antibody - Western blot analysis of E2F1 phosphorylation expression in Etoposide treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human E2F1 Antibody (phospho‑Thr433, IF, WB) LS‑C801129

Polyclonal Rabbit anti‑Human E2F1 Antibody (phospho‑Thr433, IF, WB) LS‑C801129

Antibody:
E2F1 Rabbit anti-Human Polyclonal (pThr433) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801129-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
E2F1 Rabbit anti-Human Polyclonal (pThr433) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
E2F1 antibody LS-C801129 is an unconjugated rabbit polyclonal antibody to E2F1 (pThr433) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Target
Human E2F1
Synonyms
E2F1 | E2F transcription factor 1 | PRB-binding protein E2F-1 | RBAP-1 | RBBP3 | RBBP-3 | Transcription factor E2F1 | RBAP1 | E2F-1 | PBR3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human E2F1 around the phosphorylation site of Threonine 433.
Epitope
pThr433
Specificity
Phospho-E2F1 (Thr433) Antibody detects endogenous levels of E2F1 only when phosphorylated at Threonine 433.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About E2F1
Q01094 NM_005225 NP_005216.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024