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| Catalog Number | Size | Price |
|---|---|---|
| LS-C14959-200 | 200 µg | $832 |
Monoclonal Mouse anti‑Human E2F1 Antibody (clone KH20, KH95, WB) LS‑C14959
Monoclonal Mouse anti‑Human E2F1 Antibody (clone KH20, KH95, WB) LS‑C14959
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
E2F1 antibody LS-C14959 is an unconjugated mouse monoclonal antibody to E2F1 from human. It is reactive with human and xenopus. Validated for IP and WB.
Target
Human E2F1
Synonyms
E2F1 | E2F transcription factor 1 | PRB-binding protein E2F-1 | RBAP-1 | RBBP3 | RBBP-3 | Transcription factor E2F1 | RBAP1 | E2F-1 | PBR3
Host
Mouse
Reactivity
Human, Xenopus
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
Clone
KH20, KH95
Purification
Protein G purified
Modifications
Unmodified
Immunogen
GST fusion protein corresponding to full-length human E2F1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20; aa342-386 for clone KH95.
Specificity
Recognizes human E2F-1 at 55kD. Species cross-reactivity: Xenopus.
Applications
- Western blot
- Immunoprecipitation
Usage
Western Blot Analysis: 0.5-2 ug/ml of antibody detected E2F-1 (~55kD) in a HeLa nuclear extract. Positive Antigen Control: HeLa nuclear extract. Use 20 ug per lane. Immunoprecipitation: 4 ug of a previous lot of antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About E2F1
Publications (0)
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Chimpanzee
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Reactivity:
Human, Mouse
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
