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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C14959-200 200 µg $635 
Antibody

Monoclonal Mouse anti‑Human E2F1 Antibody (clone KH20, KH95, WB) LS‑C14959

Monoclonal Mouse anti‑Human E2F1 Antibody (clone KH20, KH95, WB) LS‑C14959

Antibody:
Mouse Monoclonal to Human E2F1
Application:
WB, IP
Reactivity:
Human, Xenopus
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$635 
LS-C14959-200
200 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human E2F1
Application:
WB, IP
Reactivity:
Human, Xenopus
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
E2F1 antibody LS-C14959 is an unconjugated mouse monoclonal antibody to E2F1 from human. It is reactive with human and xenopus. Validated for IP and WB.
Target
Human E2F1
Synonyms
E2F1 | E2F transcription factor 1 | PRB-binding protein E2F-1 | RBAP-1 | RBBP3 | RBBP-3 | Transcription factor E2F1 | RBAP1 | E2F-1 | PBR3
Host
Mouse
Reactivity
Human, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
KH20, KH95
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
Immunogen
GST fusion protein corresponding to full-length human E2F1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20; aa342-386 for clone KH95.
Specificity
Recognizes human E2F-1 at 55kD. Species cross-reactivity: Xenopus.
Usage
Western Blot Analysis: 0.5-2 ug/ml of antibody detected E2F-1 (~55kD) in a HeLa nuclear extract. Positive Antigen Control: HeLa nuclear extract. Use 20 ug per lane. Immunoprecipitation: 4 ug of a previous lot of antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About E2F1
Q01094 NM_005225 NP_005216.1

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Requested From: United States
Date Requested: 7/12/2020