Human DPP2 / DPP7

Rat

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived recombinant human rhDPPII.

Recognizes human DPPII. Species sequence homology: chimpanzee 99%, mouse 81%, rat 80%, dog 79% and chicken 65%.

• Western blot (1 - 2 µg/ml)
• ELISA
• Neutralization

Suitable for use in ELISA, Neutralization and Western blot. Neutralization-preincubated with the. enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then. assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by. the antibody at approximately 0.12 ug/ml (IC50) under conditions in which the enzyme was. present at 0.1 ug/ml and the substrate concentration was 10uM. Considering the molecular. masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at. approximately 1:2 molar ratio of the antibody to the enzyme. Western blot: 1-2 ug/ml The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with. WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.

Lyophilized from PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UHL4 NCBI: NM_013379 NP_037511.2