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Antibodies > DLL4 > LS-C745290
 
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Catalog Number Size Price
LS-C745290-25 25 µl (1 mg/ml) $304 
DLL4 Antibody - Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded.
DLL4 Antibody - Western Blot of rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 µg per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.
DLL4 Antibody - Western Blot of rabbit anti-DELTA-4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate. Lane 3: Human rDLL4. Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate. Load: 10 µg per lane for Whole Cell Lysates or 50 ng for recombinant protein. Primary antibody: DELTA-4 antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 74 kDa, 74 kDa for DELTA-4. Other band(s): Not Identified.
DLL4 Antibody - ELISA results of purified Rabbit anti-DELTA-4 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
DLL4 Antibody - Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded.
DLL4 Antibody - Western Blot of rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 µg per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.
DLL4 Antibody - Western Blot of rabbit anti-DELTA-4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate. Lane 3: Human rDLL4. Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate. Load: 10 µg per lane for Whole Cell Lysates or 50 ng for recombinant protein. Primary antibody: DELTA-4 antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 74 kDa, 74 kDa for DELTA-4. Other band(s): Not Identified.
DLL4 Antibody - ELISA results of purified Rabbit anti-DELTA-4 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
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Polyclonal Rabbit anti‑Human DLL4 Antibody (Internal, IHC, WB) LS‑C745290

Polyclonal Rabbit anti‑Human DLL4 Antibody (Internal, IHC, WB) LS‑C745290

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Antibody:
DLL4 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C745290-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DLL4 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DLL4 antibody LS-C745290 is an unconjugated rabbit polyclonal antibody to human DLL4 (Internal). Validated for ELISA, IHC and WB.
Target
Human DLL4
Synonyms
DLL4 | Delta-like 4 protein | Delta like 4 | Delta-like 4 (Drosophila) | Delta-like 4 homolog | Drosophila Delta homolog 4 | Notch ligand DLL4 | Delta 4 | Delta ligand 4 | Delta-like protein 4 | Delta4 | Hdelta2 | Notch ligand delta-2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.
Epitope
Internal
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence. Cross reactivity with DELTA-4 homologues from other sources has not been determined.
Applications
  • IHC (10 - 20 µg/ml)
  • Western blot (1:500 - 1:2500)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DLL4
Q9NR61 NM_019074 NP_061947.1

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Featured Products

DLL4 Antibody - Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
DLL4 Antibody (Internal) LS‑C19035
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
DLL4 Antibody - IHC of paraffin-embedded rectum cancer tissues using DLL4 mouse monoclonal antibody with DAB staining.
DLL4 Antibody (clone 4A11F8) LS‑C169100
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
DLL4 Antibody - IHC with DLL4 antibody on frozen human tonsil. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DLL4 Antibody (aa146‑195) LS‑B10026
Species: Human
Applications: IHC, IHC - Frozen, Western blot
Product
DLL4 Antibody (aa27‑525, Biotin) LS‑C125452
Species: Mouse
Applications: ELISA
DLL4 Antibody - Western blot of DLL4 in HUVEC cell lysate using 1) preimmune sera at 1:5000 and 2) Polyclonal Antibody to DLL4 at 3 ug/ml, and 3) mouse embryo body lysate at 6 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
DLL4 Antibody (aa550‑600) LS‑C148713
Species: Human, Mouse
Applications: Western blot
Product
DLL4 Antibody (clone 12G28) LS‑C182616
Species: Mouse
Applications: IHC, Flow Cytometry
See all DLL4 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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