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Catalog Number	Size	Price
LS-C408014-10	10 µg	$318
LS-C408014-100	100 µg	$470

DLG4 / PSD95 Antibody - PSD95 antibody IHC-paraffin. IHC(P): Human Glioma Tissue.

DLG4 / PSD95 Antibody - Western blot analysis of PSD95 using anti-PSD95 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: mouse brain Tissue Lysate, Lane 3: human SHG-44 Whole Cell Lysate, Lane 4: human U-87MG Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD95 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSD95 at approximately 100KD. The expected band size for PSD95 is at 80KD.

DLG4 / PSD95 Antibody - PSD95 antibody Western blot. All lanes: Anti PSD95 at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: A549 Whole Cell Lysate at 40 ug. Lane 5: 293T Whole Cell Lysate at 40 ug. Predicted band size: 95 kD, 85 kD. Observed band size: 95 kD, 85 kD.

DLG4 / PSD95 Antibody - Flow Cytometry analysis of A431 cells using anti-PSD95 antibody. Overlay histogram showing A431 cells stained with anti-PSD95 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD95 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human DLG4 / PSD95 Antibody (aa581‑724, IHC, WB) LS‑C408014

Name: Anti-DLG4 / PSD95 Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C408014-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

DLG4 / PSD95 Rabbit anti-Human Polyclonal (aa581-724) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

$318

LS-C408014-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

DLG4 / PSD95 Rabbit anti-Human Polyclonal (aa581-724) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

PSD95 antibody LS-C408014 is an unconjugated rabbit polyclonal antibody to PSD95 (DLG4) (aa581-724) from human. It is reactive with human and rat. Validated for IHC and WB.

Target

Human DLG4 / PSD95

Synonyms

DLG4 | Disks large homolog 4 | Presynaptic density protein 95 | PSD95 | SAP-90 | Synapse-associated protein 90 | PSD-95 | SAP90 | Tax interaction protein 15 | Discs large homolog 4

See All DLG4 / PSD95 Antibodies

Host

Rabbit

Reactivity

Human, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human PSD95 recombinant protein (Position: H581-L724). Human PSD95 shares 100% amino acid (aa) sequence identity with both mouse and rat PSD95.

Epitope

aa581-724

Specificity

Brain.

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About DLG4 / PSD95

Uniprot: P78352 NCBI: NM_001365 NP_001356.1