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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DFFB Antibody (aa205‑222, clone 36A349) LS‑C534
DFFB antibody LS-C534 is an unconjugated mouse monoclonal antibody to human DFFB. Validated for WB.
Catalog
Size
Price
LS-C534-100
100 µg (0.5 mg/ml)
$335

Popular DFFB Products

Anti-DFFB antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B559 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-DFF40 / DFFB antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7893 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 µg/ml.
Species: Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 µg/ml.
Species: Mouse
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemical of paraffin-embedded H.kidney section using DFFB Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

DFFB antibody LS-C534 is an unconjugated mouse monoclonal antibody to human DFFB. Validated for WB.

Specifications

Target
Human DFFB
Synonyms
DFFB, Caspase-activated nuclease, DFF-40, DFF40, Caspase-activated DNase, CPAN, DFF2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [36A349]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (2 µg/ml)
Immunogen
DFFB antibody was raised against a peptide corresponding to amino acids 205 to 222 of human DFF40 (3).
Epitope
aa205-222
Specificity
In HeLa, a 40 kD band is observed.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About DFFB
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). O76075 NM_004402 NP_004393.1


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Images

Western blot

Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.
Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.

Western blot

Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.
Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.

Western blot

Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.
Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.

Western blot

Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.
Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.

Requested From: United States
Date Requested: 10/16/2018

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