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Catalog Number Size Price
LS-C344928-100 100 µg (1 mg/ml) $505 
DDX58 / RIG-1 / RIG-I Antibody - Immunohistochemical staining of endogenous human RIG-I in different human tissues (paraffin sections) using anti-RIG-I, mAb (Alme-1) . .Different human normal tissues (A: Nasopharynx; B: Colon; C: Endometrium) or cancer tissues (D: Breast; E: Skin; F: Ovarian) were stained with anti-RIG-I, mAb (Alme-1) by standard immunohistochemistry.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DDX58 / RIG-1 / RIG-I Antibody - Western blot analysis of human RIG-I in HeLa cells by using RIG-I, mAb (Alme-1) . Method: Cell extracts from HeLa cells either unstimulated (lane 1) or stimulated for 6h (lane 2), 16h (lane 3) or 24 h (lane 4) with Interferon gamma were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the RIG-I , mAb (Alme-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DDX58 / RIG-1 / RIG-I Antibody - Immunohistochemical staining of endogenous human RIG-I in different human tissues (paraffin sections) using anti-RIG-I, mAb (Alme-1) . .Different human normal tissues (A: Nasopharynx; B: Colon; C: Endometrium) or cancer tissues (D: Breast; E: Skin; F: Ovarian) were stained with anti-RIG-I, mAb (Alme-1) by standard immunohistochemistry.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DDX58 / RIG-1 / RIG-I Antibody - Western blot analysis of human RIG-I in HeLa cells by using RIG-I, mAb (Alme-1) . Method: Cell extracts from HeLa cells either unstimulated (lane 1) or stimulated for 6h (lane 2), 16h (lane 3) or 24 h (lane 4) with Interferon gamma were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the RIG-I , mAb (Alme-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Monoclonal Mouse anti‑Human DDX58 / RIG‑1 / RIG‑I Antibody (clone Alme‑1, aa201‑713, IHC, WB) LS‑C344928

Monoclonal Mouse anti‑Human DDX58 / RIG‑1 / RIG‑I Antibody (clone Alme‑1, aa201‑713, IHC, WB) LS‑C344928

Antibody:
DDX58 / RIG-1 / RIG-I Mouse anti-Human Monoclonal (aa201-713) (Alme-1) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$505
LS-C344928-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DDX58 / RIG-1 / RIG-I Mouse anti-Human Monoclonal (aa201-713) (Alme-1) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
RIG-I antibody LS-C344928 is an unconjugated mouse monoclonal antibody to RIG-I (DDX58 / RIG-1) (aa201-713) from human. It is reactive with human and mouse. Validated for IHC, IP and WB. Cited in 1 publication.
Target
Human DDX58 / RIG-1 / RIG-I
Synonyms
DDX58 | RIG-1 | RIG-I | RLR-1 | Retinoic acid inducible gene I | RIG-I-like receptor 1 | RIGI | DEAD box protein 58 | RNA helicase RIG-I
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
Alme-1
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant human RIG-I (aa 201-713).
Epitope
aa201-713
Specificity
Recognizes human and mouse RIG-I.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:1000)
  • Immunoprecipitation (1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry - Paraffin sections. Immunoprecipitation - 1:200. Western blot - 1:1000.
Presentation
PBS, 0.02% Sodium Azide, 10% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDX58 / RIG-1 / RIG-I
O95786 NM_014314 NP_055129.2

LSBio Ratings

DDX58 / RIG-1 / RIG-I Antibody (aa201-713, clone Alme-1) for IHC, WB/Western, IP LS-C344928 has an LSBio Rating of
Publications (4)

Publications (1)

Mechanism of Nucleic Acid Sensing in Retinal Pigment Epithelium (RPE): RIG-I Mediates Type I Interferon Response in Human RPE. Joshua Schustak, Michael Twarog, Xiaoqiu Wu, Henry Y Wu, Qian Huang, Yi Bao. Journal of immunology research. 2021 Jun;2021:9975628.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/25/2024