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Catalog Number Size Price
LS-B3768-50 50 µg (0.5 mg/ml) $485 
DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
DDX58 / RIG-1 / RIG-I Antibody - DDX58 / RIG-1 / RIG-I antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DDX58 / RIG-1 / RIG-I Antibody - DDX58 / RIG-1 / RIG-I antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DDX58 / RIG-1 / RIG-I Antibody - DDX58 / RIG-1 / RIG-I antibody flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
DDX58 / RIG-1 / RIG-I Antibody - DDX58 / RIG-1 / RIG-I antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DDX58 / RIG-1 / RIG-I Antibody - DDX58 / RIG-1 / RIG-I antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DDX58 / RIG-1 / RIG-I Antibody - DDX58 / RIG-1 / RIG-I antibody flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human DDX58 / RIG‑1 / RIG‑I Antibody (C‑Terminus, IHC, IF) LS‑B3768

IHC‑plus™ Polyclonal Goat anti‑Human DDX58 / RIG‑1 / RIG‑I Antibody (C‑Terminus, IHC, IF) LS‑B3768

Note: This antibody replaces LS-C108160
Antibody:
DDX58 / RIG-1 / RIG-I Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IF, Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B3768-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DDX58 / RIG-1 / RIG-I Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IF, Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RIG-I antibody LS-B3768 is an unconjugated goat polyclonal antibody to human RIG-I (DDX58 / RIG-1) (C-Terminus). Validated for Flow, IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human DDX58 / RIG-1 / RIG-I
Synonyms
DDX58 | RIG-1 | RIG-I | RLR-1 | Retinoic acid inducible gene I | RIG-I-like receptor 1 | RIGI | DEAD box protein 58 | RNA helicase RIG-I
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KDFHFEKIPFDPAE, from the C-Terminus of the protein sequence according to NP_055129.2.
Epitope
C-Terminus
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml): Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells.
  • Flow Cytometry (10 µg/ml): U937 cells.
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000): Detection Limit
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDX58 / RIG-1 / RIG-I
O95786 NM_014314 NP_055129.2

Validation

DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-DDX58 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-DDX58 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-DDX58 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-DDX58 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

DDX58 / RIG-1 / RIG-I Antibody - Anti-DDX58 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-DDX58 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-DDX58 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LSBio Ratings

IHC-plus™ DDX58 / RIG-1 / RIG-I Antibody (C-Terminus) for IHC, IF/Immunofluorescence, Flow LS-B3768 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission. Giugliano S, Petroff MG, Warren BD, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko MA, Gale M Jr, Golden-Mason L, Winn VD, Rosen HR. Journal of immunology (Baltimore, Md. : 1950). 2015 195:3737-47. (WB, IHC-P; Human)

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Requested From: United States
Date Requested: 4/24/2024